Researchers from the Royal Victoria Hospital, Montréal, Canada, recently published a study in the Endocrine journal showing that the role of the Parathyroid Hormone-Related Protein (PTHrP) in melanoma invasion and metastasis is closely associated with tumour progression.
The action mechanism of this molecule was studied through in vitro inactivation of the Pthrp gene in human melanoma cell lines, to understand if inactivating the Pthrp gene could be beneficial to brake melanoma progression.
Pthrp-ablated cells showed rounder morphology, decreased motility and a significant reduction in the invasion capacity compared with nonablated A375 cells. PTHrP peptide and culture medium from the melanoma cell line restored, partially, the invasive phenotype of the genetic-deficient cells. Furthermore, the team observed that Pthrp ablation decreased actin polymerization, matrix metallopeptidase 9 expression and focal adhesion kinase phosphorylation.